Association of Serum Omentin-1 Levels with Coronary Artery Disease (CAD) in a local population of Karachi - A Multicenter Study

Abstract

Objective: To  determine  the  association  between  circulating  levels  of  omentin-1,  a  novel adipocytokine and coronary artery disease in the local population of Karachi.

Methods: A total of 350 subjects were included  in  the  study  from  June  2016  to  December  2016.  About 250 patients, who had undergone coronary angiography in Karachi Institute  of  Heart  Disease (KIHD) and Civil Hospital Karachi (CHK) and 100 served as healthy controls. Serum concentrations of omentin-1 were measured by using enzyme-linked immunosorbent assay (ELISA) at Dr. A Q. Khan In- stitute of Biotechnology (KIBGE) and lipid profile, fasting  blood  sugar  were  done  at  KIHD  lab.  Coro- nary artery disease (CAD) and cardiovascular disease  risk  factors  were  evaluated  by  using  the  software SPSS 16. Independent sample t-test and chi-square tests were used to analyse the clinical and demographic characteristics  of  data.  Simple  logistic  regression  and  then  multivariate  analysis  were performed to evaluate correlations between CAD and all other parameters. P value less than

0.05 was considered statistically significant. This research is part of MPhil thesis and there  are  two research papers; (1) Serum Omentin-1 Levels with CAD (current paper) and (2) genetic polymor- phism/mutation of omentin-1 gene in  developing  CAD.  Latter  is  also  in  process  of  publication.

Results:  Serum omentin-1 levels were found to be lower in patients with CAD as compared to controls (456 ± 99 vs  739  ±  72;  p<0.01).  Serum  concentration  of  omentin-1  was  negatively  correlated with body waist circumference (39.95 ± 3.1  vs  30.95  ±  3.7;  p<  0.05),  whereas  positively  associated with serum High Density Lipoprotein (HDL) levels (25.9 ± 6.2 vs 42.3 ± 10; p< 0.05). Moreover, multiple logistic regression  analysis  demonstrated  that  serum  omentin-1  concentration  was  indepen-  dently correlated with CAD.

Conclusion: The current study has suggested that  serum  concentrations  of  omentin-1  might  be  re- lated to pathogenesis and progress of CAD.